ABSTRACT
Objective: To establish the HPLC fingerprint of liquid of raw material-intermediate product-Yinju Jiedu Oral Liquid (YJOL), and to determine the multi-components in YJOL, thus to provide an approach and basis for the quality control in production. Methods: The separation was performed on Shimadzu Inert Sustain C18 columin (250 mm × 4.6 mm, 5 μm) with mobile phase composed of acetonitrile-0.1% phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL/min, the column temperature was set at 30 ℃ and the detection wavelengths were set at 240, 327, 334, and 280 nm. HPLC fingerprints of the extracts from 12 batches of raw material, intermediate product, and YJOL were established and compared. The content was determined and the common peaks were identified, and some of the characteristic peaks were analyzed. Results: Fifteen common peaks of intermediate product and liquid of raw material and 13 common peaks in YJOL were determined. The similarities of YJOL were over 0.9, the same with the similarities of the liquid of raw material and intermediate product. The correlation was good between the extracts from the liquid of raw material, intermediate product and YJOL. Peak 1 was from Isatidis Radix, peaks 2-7 were from Honeysuckle of Sichuan and Chrysanthemum indicum, peaks 8 and 9 were from Chrysanthemum indicum, peaks 10 and 11 were from Scrophulariae Radix, peaks 12 and 13 were from Scurfpea fruit. Based on the retention time of master compounds, six components [R, S-epigoitrin (peak 1), chlorogenic acid (peak 3), linarin (peak 10), harpagoside (peak 11), psoralen (peak 13), and psoralen (peak 14)] were identified and quantified. The average contents of six components were 39.21, 15.43, 2.14, 0.53, 7.21, and 6.51 mg/g in raw material, were 35.31, 11.54, 1.83, 0.37, 4.95, and 4.74 mg/g in intermediate product, and were 32.87, 10.58, 1.72, 0.31, 4.58, and 4.48 mg/g in YJOL. Conclusion: This validated method is suitable for the quality evaluation and quality control of YJOL.
ABSTRACT
Objective To multi-objectively optimize the purification process parameters of Yinju Jiedu Oral Liquid (YJOL), mixed weight of AHP-CRITIC, Plackett-Burman design (PBD), and Box-Behnken design (BBD) were adopted on the basis of HPLC fingerprints. Methods HPLC was used to establish HPLC fingerprint of YJOL. Recovery rate of fingerprints of six components (chlorogenic acid, linarin, harpagoside, R,S-epigoitrin, psoralen, and isobalin) and HPLC fingerprint similarity was taken as the index to optimize the type of macroporous resin among 10 types. Weights of the recovery rates of six components and similarity were determined by mixed weight of AHP-CRITIC, in order to obtain the comprehensive index as the evaluation criterion. The significantly influencing factors were firstly evaluated by PBD, and purification conditions were optimized by BBD. Results HPD-400 type resin showed a high selectivity for six components. The optimized purification technology was as follows: the ratio of dia-height was 1:7, pH value was 3.5, sample concentration was 0.18 g/mL, ratio of sample to resin was 0.96 g/g, and 77% ethanol's dosage was 8 BV. Under the conditions, the recovery rates of six components were 78%-98%, and the similarity of fingerprint was higher than 0.99. These components could get balanced recovery. Moreover, the theoretical and actual comprehensive indexes were 94.28% and 93.69%, respectively, with a relative error of 0.59%. Conclusion Based on HPLC fingerprints, mixed weight of AHP-CRITIC, combined with PBD and BBD-RSM used to optimize the purification process for the YJOL in this study is scientific and feasible, this way can improve the purity of the active components and keep the uniformity of main components in the YJOL as well.
ABSTRACT
<p><b>OBJECTIVE</b>The purposes of this thesis were to study the behavior about workers exposed to dust and provide scientific basis for health promotion.</p><p><b>METHODS</b>We designed a questionnaire and carry it on the 746 dust workers in the 3 representative corporations of Machinery, Ceramic, and Metallurgy Industry. All data were input into computer. And a database was established with Excel. SPSS11.5 statistical analysis software was used to analyze the influence on protecting behavioral between the application of qualifications, different jobs, training or protection, and other aspects etc.</p><p><b>RESULTS</b>The rates were 94.4% and 75.3% about the regular physical examination and requirements for protective equipment. The rate of choosing an effective way of protection was generally low (15.4%). There was significant difference for among different educational background workers (P < 0.01). The rates of choosing an effective way of protection (20.3%), the regular physical examination (98.3%) and requirements for protective equipment (86.4%) in the dust workers who participated in the training of dust protection were superior than those who did not participated in the training. There was the significant difference (P < 0.05, P < 0.01). There was the significant difference for the rate of effective way of protection, regular physical examination, and requirements for protective equipment among the different corporations (P < 0.05).</p><p><b>CONCLUSIONS</b>Dust workers' using rate about the choosing an effective way of protection was generally low in Machinery, Ceramic, and Metallurgy Industry. Those who were not educated had a lower using rate about the protection behavior, regular physical examination, and requirements for protective equipment than those educated.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Ceramics , Choice Behavior , Dust , Industry , Metallurgy , Occupational Exposure , Respiratory Protective DevicesABSTRACT
To produce antibodies capable of neutralizing botulinum neurotoxin type B(BoNT/B),We cloned the carboxy-terminal end of Hc containing the major determinants responsible for specific toxin,induced and purifed.The heavy-chain and kappa light-chain variable region gene repertoire of immunoglobulin were amplified individually from the spleen cell mRNA by RT-PCR and joined as a single-chain Fv(scFv)DNA fragment.These fragment were cloned into the phagemid pCANTAB5E and the phage display library was constructed.Results showed that the high affinity scFv was obtained after 4 rounds of panning,with its DNA sequence conforming to that of mouse antibody.